Sciclone V3.0 Software Demonstrations

Chose lesson's/demonstrations files currently available on the links below. These tutorials will give you an impression how easy it is to program the Sciclone ALH 3000 robot. The software is truly user-friendly with intuitive user interface
that mimics the familiar outlook GUI.

THESE TUTORIAL'S IS RATHER OLD, LATEST SOFTWARE VERSION IS 3.4 AND BIG IMPROVEMENT HAS BEEN DONE . THE SOFTWARE IS STILL EASY TO USE BUT MORE POWERFUL WITH A LOT MORE FEATURES BUILT IN.

Creating a layout.

In this video, a layout is created to do a reformatting application.  Four 96–well plates,a single 384-well plate
a reservoir, two array parking stations, and wash stations are addedto the Sciclone Deck.  The names and/or
material types are changed as needed for each.  Features are pointed out with annotations. 

Creating an application.

In this video, the layout created in the Creating a Layout Video is used to build a reformatting application. 
A 96-Cannula Array is picked up and used to transfer solution from each Source Plate to each quadrant o
f the 384-well Assay Plate.  The 96-Cannula Array is then swapped for a 384-Cannula Array.  This array is used
to transfer a reagent from the reservoir to the assay plate and mix.  The array is finally parked.  Steps and
Features are annotated.

Bulkdispensing using a local variable.

In this video, the Bulk Dispenser is used to add 20uL to every well of a 96-well plate.  A Local Variable, which
can be numeric, string (text), or Boolean (true/false) and can only be used within a single method, is defined
as Column Number.  A Begin…End Loop is used to step from column 1 to column 12 of the 96-well plate. 
Global Variables, which can be used between methods within a single application, and Parameters, which
can be set at run time by the user and are used within a single method, are pointed out.  Features regarding
Bulk Dispensing are annotated.

Using parameters in Sciclone software ver.3.xx.

In this video, 2 Parameters are defined and used with the Bulk Dispense Method created in the Bulk Dispense Video. 
These Parameters, Blank Column and Blank Column Vol, are given values by the user at run time.  An If…Then statement
is used within the Begin..End Loop to alter the dispense volume for a single column.  The User can define which
Column is the control/blank column and specify a different volume for that one column.  A second method is created.
 This method calls Method 1, showing the Parameters are defined as part of the Run Method Command. 

Working with liquidclasses.

In this video, a new liquid class is created.  A liquid class contains the syringe calibration for a specific aspirate speed,
dispense speed, and pre/post air gap for a specific liquid type (ie. Ethanol, 1M Acetic Acid, etc).  It also contains minimum
and maximum volumes for the device used (Low Volume Head, High Volume Head, Bulk Dispenser, etc) and the
units (uL, mL, nL).  A simulated calibration is performed to show features.